ABSTRACT
AIM:To investigate the role of Bcl-2-associated athanogene 2(BAG2)in the proliferation of human lung adenocarcinoma A549 cells and its clinical implications.METHODS:The abundance of BAG2 protein in A549 and lung bronchial epithelium(HBE)cells were measured by Western blot.After down-regulation of BAG2 by transfection of siRNA in A549 cells, the expression of cell proliferation and cell cycle related proteins were detected by CFSE assay, WST-1 assay and Western blot,respectively.Moreover,the expression of BAG2 in cDNA array which contained 10 pairs of lung cancer and adjacent tissue was verified.Meanwhile, BAG2 expression in GEO database, which included the human lung cancer and adjacent tissue microarray data was analyzed.The prognosis power of BAG2 was evaluated by the Kaplan-Meier survival curve analysis.RESULTS:BAG2 had remarkably higher expression level in A 549 cells than that in HBE cells.Knockdown of BAG2 resulted in significantly inhibition of proliferation in A 549 cells,accompany with the significant-ly down-regulation of cyclin B1 and cyclin E1.BAG2 was over-expressed in the lung cancer tissues,as compared with the adjacent normal tissues.Kaplan-Meier plotter and cDNA microarray results showed that patients with higher BAG 2 expres-sion were significantly associated with poorer survival.CONCLUSION:The BAG2 gene tends to regulate A549 cells pro-liferation via cyclin B1 and cyclin E1.BAG2 has significantly prognostic power on the survival of lung cancer patients.
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the differential proteomics in human umbilical cord mesenchymal stem cells (MSC) induced by chemical hypoxia-mimetic agent cobalt chloride (CoCl(2)) by two-dimensional gel electrophoresis (2-DE) and mass-spectrometry.</p><p><b>METHODS</b>2-DE was performed to separate proteins from treated and untreated human umbilical cord MSC with CoCl(2). 2-DE images were analyzed by ImageMaster 2D Platinum software 6.0. The differential expressed proteins was identified by MALDI-TOF-MS. The differential proteins were classified based on their functions.</p><p><b>RESULTS</b>2-DE reference patterns of CoCl(2) treated human umbilical cord MSC were established. A total of twenty-six differential proteins were identified, of them eleven proteins were up-regulated and fifteen down-regulated. Their biological functions involved in carbohydrate metabolism, protein metabolism and modification, lipid metabolism, coenzyme and prosthetic group metabolism, cell cycle, immunity and defense, cell structure and motility, signal transduction, protein targeting and localization, neuronal activities, muscle contraction, etc. Peroxiredoxin1 (Prdx) was down-regulated, whereas alpha-enolase (ENO1) and vesicle amine transport protein 1 homolog (VAT1) up-regulated.</p><p><b>CONCLUSION</b>The effects of hypoxia on human umbilical cord MSC were participated by multiple proteins and involved in multiple functional pathways.</p>
Subject(s)
Humans , Cobalt , Pharmacology , Mesenchymal Stem Cells , Metabolism , Proteome , Proteomics , Umbilical Cord , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To establish methods for quantitative determination of ginseng saponins, ginsenoside Rg1, Re, Rb1 and polysaccarides and compare the qualities of Tongrentang Red Ginseng and Korean Red Ginseng.</p><p><b>METHOD</b>Macroreticular resin-colorimetric method was developed to determine ginseng saponins and a new HPLC method with gradient eluents was established for determination of ginsenoside Rg1, Re, Rb1. For ginseng polysaccharides, phenol-oil of vitriol colorimetric method was developed and some factors were also optimized.</p><p><b>RESULT</b>The content of ginseng saponins in Tongrentang Red Ginseng was not lower than that of Korean Red Ginseng. Ginsenoside Rg1 and Rb1 in Tongrentang Red Ginseng were higher than those in Korean Red Ginseng, while Ginsenoside Re was slightly lower than that of Korean Red Ginseng. However, the amount of Ginseng Polysaccharides in Tongrentang Red Ginseng was greater than those in Korean Red Ginseng.</p><p><b>CONCLUSION</b>The contents of ginseng saponins and ginsenoside Rg1, Re, Rb1 in Tongrentang Red Ginseng were not lower than that in Korean Red Ginseng. The methods for determination of ginsenosides and ginseng polysaccharides were quite accurate and reliable to the quality control of Ginseng.</p>